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The cell pellet was lysed in 1 ml of lysis buffer containing 10 mM Tris HCl (pH 7.5), 150 mM NaCl, 5 mM EDTA, 0.5% Triton X-100, 1 mM phenylmethylsulfonyl fluoride, and 1 mM dithiothreitol (DTT), sonicated at 4 C using ultrasonic homogenizer Q700 (QSONICA), and then centrifuged at 17,200 g for 5 min at 4 C

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Surg Clin North Am 99(2):215229 Ngashangva L, Bachu V, Goswami P (2019Jan) Development of new methods for determination of bilirubin