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Step 4: Rinse thoroughly with water and pat dry
Weller and Borondy, 1967
Finally, these sulfur-seeking metals and other chemicals have been shown to directly remove reduced glutathione (GSH) from the cell, as well as inhibit the activities of two key enzymes involved in GSH metabolism: GSH synthetase and GSH reductase (Quig, 1998
Cells were imaged in fresh FluoroBrite DMEM supplemented with 4 mM glutamine, 1 mM sodium pyruvate, 10% FBS and penicillin (100 U ml 1 )/streptomycin (100 g ml 1 )/fungizone (0.25 g ml 1 )
This effect was suppressed after PRMT1 knockdown (Fig
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