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Ma EH, Bantug G, Griss T, Condotta S, Johnson RM, Samborska B, et al
New England Journal of Medicine, 357, 23592370
Therefore, we sought evidence that in mature human intervertebral discs, matrix disruption and swelling can disturb cell-matrix binding and lead to cell clustering, together with expression of a degenerative cell phenotype
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Primary antibodies were incubated for 60 min at room temperature (thioredoxin 1:1000 (#705, American Diagnostica, Stamford, USA), thioredoxin reductase 1:1000 (#07-613, Millipore, Billerica, USA), TxNIP 1:3000 (clone JY2, MBL International Corporation, Woburn, USA), catalase 1:5000 (#ab1877, Abcam, Cambridge, UK), MnSOD 1:1000 (#ab13533, Abcam), GST (clone 2F7) 1:333, GST (clone 2E10-1B2) 1:500 (Abnova, Taipei City, Taiwan), GST 1:2000 (#MSA-102, Assay Designs, Michigan, USA))
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