Western blot analysis Preparations of cell lysates and Western blot cells or tissues were lysed in RIPA buffer (phosphate-buffered saline containing 1% Nonidet P-40, 0.1% sodium dodecyl sulfate [SDS], 1 mmol/L Na3VO4, 4 mm phenylmethylsulfonyl fluoride and 0.05% [w/v] apro-tinin)
Xie X, Zhong M, Huang X, Yuan X, Mahna N, Mussagy CU, Ren M (2024) Astaxanthin biosynthesis for functional food development and space missions
The reactions were performed in 200 L ice-cold assay buffer containing 1 mg/mL cell lysate protein, 0.5% CHAPS, 2 mM NADH and 100 M K vitamins at 30 C for 1 h
Methionine restriction in lower organisms has been shown to extend lifespan
This helps restore balance, enhance energy, and improve overall wellness