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The slides were treated with a lysis solution (2.5 M NaCl, 100 mM EDTA, 100 mM TRIS, 1% Triton X-100, 10% DMSO, pH ~ 10.2) for 24 h at 4C

Matsuoka H, Hayashi T, and Takigami K, et al (2020) Correlation between immune-related adverse events and prognosis in patients with various cancers treated with anti PD-1 antibody BMC Cancer 20 (1) 656 PMID: 32664888 PMCID: 7362440

The formulation combines GHK-Cu, BPC-157 and TB-500 in a high-purity lyophilized preparation designed for controlled in vitro experimentation