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Using sterile water (which has no preservative) means your vial is susceptible to bacterial growth the moment you make that first puncture

Scappaticcio, P

Based on these parameters, slides were scored on a 03 scale (0 = normal, 1 = mild, 2 = moderate, and 3 = severe disease)

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doi: 10.1016/j.isci.2022.105648 280 XieJBruggemanADe NolfCVandendriesscheCVan ImschootGVan WonterghemEet al