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Mann-Whitney u test and Wilcoxon Ranks test was used for comparing the nonnormal distributed continuous variables between groups and within groups pre and postintervention

In other studies, prophylactic immunization with the hydatid cyst wall protein bands (~ 27/28 kDa), that showed cross-reactivity with breast cancer patients sera, or crude hydatid cyst wall antigens (laminated layer) caused significant inhibition of 4T1 breast tumor growth, decrease of metastasis, and prolongation of the tumor-bearing mice survival time through a rise in the levels of IL-2, TNF-, IFN-, and IL-4 [51, 181]