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Organoids were stimulated as described above for 24 h, washed with PBS and fixed with 4% PFA at room temperature for 20 min, washed again twice with PBS and permeabilized with PBS-Triton X 0.5% for 20 min at room temperature

do not inject directly onto the lyophilized cake and do not shake the vial, as mechanical agitation promotes aggregation in peptides

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