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The importance of early avoidance or treatment of sepsis, anti-inflammatory strategies, nutritional support and subsequent rehabilitation is emphasized in previous studies

When working with an eukaryotic expression system that may contain unknown agents and factors interfering the binding of target protein, it is recommended to dilute the culture supernatant 1:1 or 1:2 with the Equilibration buffer (for example, 20 mM sodium phosphate, 0.5 M NaCl, pH 7.4)

Together, they form the gold standard for diagnosis