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Four hours later, cells were washed with PBS and detached with TrypLE (Gibco)

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Mouse interleukin (IL)-6 and mouse tumor necrosis factor-alpha (TNF-) levels in cell culture media samples were measured utilizing BioLegends corresponding ELISA MAX Deluxe Sets (431315 and 430915, San Diego, CA, USA) in accordance with the manufacturers protocol

We are running experiments where a known concentration of TMA is mixed with a standard volume of probiotic solution

Therefore, markers of inflammation, hematoma, edema, and the increase in bladder weight were analyzed after 24 h of CYP exposure