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[4] [6] The proposed mechanism involves rapid weight loss leading to changes in bile composition and gallbladder motility

Additionally, NRF2 regulates the expression of enzymes such as glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), malic enzyme 1 (ME1), and isocitrate dehydrogenase 1 (IDH1), which are involved in generating reduced nicotinamide adenine dinucleotide phosphate (NADPH), a crucial cofactor for antioxidant reactions (Wu et al

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the lysate supernatant was incubated with 1 ml of Ni-NTA Superflow resin (QIAGEN) for 1 h at 4 C