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The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2

Beyond-use dates and storage conditions must be determined by validated stability data for the specific product, not by general estimates

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Frequent or high-dose use can increase oxidative stress and, in some cases, cause liver injuryespecially when combined with alcohol or preexisting liver strain