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Abbreviations 16S rRNA: 16S Ribosomal RNA ABS: Acrylonitrile Butadiene Styrene ANOVA: Analysis of Variance ATP: Adenosine Triphosphate ATP6: ATP Synthase F0 Subunit 6 BCS: Body Condition Score BSA: Bovine Serum Albumin BW: Body Weight CD44: Cluster of Differentiation 44 CD45: Cluster of Differentiation 45 CD8: Cluster of Differentiation 8 cDNA: Complementary DNA CNS: Cresty Neck Score COX1: Cytochrome C Oxidase Subunit 1 COX2: Cytochrome C Oxidase Subunit 2 COX3: Cytochrome C Oxidase Subunit 3 COX4L1: Cytochrome C Oxidase Subunit 4L1 COX4L2: Cytochrome C Oxidase Subunit 4L2 CRP: C-Reactive Protein DNA: Deoxyribonucleic Acid DMEM-LG: Dulbeccos Modified Eagles MediumLow Glucose EMS: Equine Metabolic Syndrome EqASC EMS : Adipose-Derived Stem Cells from Horses Affected by EMS EqASC EMS + 50 M SHBG: Adipose-Derived Stem Cells from Horses Affected by EMS Treated with 50 M SHBG EqASC HE : Healthy Adipose-Derived Stem Cells FACS: Flow Cytometry FBS: Fetal Bovine Serum FOXO1: FKHR-Forkhead in Rhabdomyosarcoma GAPDH: Glyceraldehyde 3-Phosphate Dehydrogenase GDM: Gestational Diabetes Mellitus GLUT4: Glucose Transporter 4 Gdna: Genomic DNA IFN-: Interferon- IL-1: Interleukin 1 Beta IL-10: Interleukin 10 IL-13: Interleukin 13 IL-4: Interleukin 4 IL-6: Interleukin 6 IR: Insulin Resistance LDH: Lactate Dehydrogenase MFN-1: Mitofusin 1 MIEF: Mitochondrial Elongation Factor 1 MIRO-1: Ras Homolog Family Member T1 (RHOT1) M1: Pro-Inflammatory Macrophages M2: Anti-Inflammatory Macrophages MMP: Mitochondrial Transmembrane Potential mtDNA: Mitochondrial DNA MTERF4: Mitochondrial Transcription Termination Factor 4 NAD: Nicotinamide Adenine Dinucleotide NADH: Reduced Nicotinamide Adenine Dinucleotide M: Nanomole NK: Natural Killer Cells OXA1L: Mitochondrial Inner Membrane Protein OXPHOS: Oxidative Phosphorylation P/S: Penicillin/Streptomycin PARKIN: Parkin RBR E3 Ubiquitin Protein Ligase (PRKN) PBS: Phosphate-Buffered Saline PCR: Polymerase Chain Reaction PFA: Paraformaldehyde PINK: PTEN Induced Putative Kinase 1 PVDF: Polyvinylidene Difluoride RNA: Ribonucleic Acid ROS: Reactive Oxygen Species SDS-PAGE: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis SHBG: Sex Hormone Binding Globulin TBST: Tris Buffered Saline With Tween-20 T2DM: Type 2 Diabetes Mellitus TNF-: Tumor Necrosis Factor Alpha UCP-1: Uncoupling Protein 1 Wnt-3: Wnt Family Member 3 References Frank N

PRF is created by collecting a small sample of the patients blood and processing it in a centrifuge to concentrate the platelets and growth factors

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Ethacrynic acid has anti-inflammatory properties that reduces the retinoid-induced ear edema in mice