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Abstract Background: Vitamin B12 deficiency is a widespread condition, particularly among elderly individuals, patients with malabsorption syndromes, and those following plant-based diets
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Ice crystal formation denatures the peptide irreversibly Unreconstituted powder (lyophilized): - Store in the freezer at -20 degrees Celsius for maximum shelf life (months to years) - Refrigerated storage at 2 to 8 degrees Celsius is acceptable for several months - Room temperature tolerance: 2 to 4 weeks without significant degradation - Keep the desiccant packet in the container to absorb moisture Practical storage tips: - Store vials on a middle shelf of the refrigerator, not in the door
Lab follow-up helps show whether the schedule is correcting deficiency and whether ongoing maintenance is needed
Light Degradation: Photooxidation can change the primary, secondary, and tertiary structures of proteins and lead to differences in long-term stability, bioactivity, or immunogenicity (19)
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