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Two endangered insular subspecies of the mainland leopard cat ( Prionailurus bengalensis ), Iriomote cats ( Prionailurus bengalensis iriomotensis ) and Tsushima leopard cats ( Prionailurus bengalensis euptilurus ) (Figures 1A,B), are endemic to Japan with an estimated population size of about 100 individuals each ( Figure 1 The distributions of Iriomote cats and Tsushima leopard cats are limited to small Japanese islands, Iriomote-jima Island (24 20 N 123 48 E, 284 km 2 ), and Tsushima Islands (34 25 N 129 20 E, 708.7 km 2 ), respectively (Figure 1C)

1 for schematic overview of sample collection and processing procedures.) The roles of these preparation conditions and their respective hypothesis-testing comparisons are detailed in Table 1

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Absorbing the upper plasma into another tube add with same volume Reagent I

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