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Cell Viability Assays Cell were treated in 96-well plate for 48 h and incubated with 5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) for 4 h at 37 C in the dark

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J Nutr Biochem 2024:109622 Janczewski (2022) Sulforaphane and its bifunctional analogs: synthesis and biological activity

Key data point: in SURMOUNT-1, tirzepatides dual GIP/GLP-1 action produced about 21 percent mean weight loss at 72 weeks

Partial AsGGT1 , AsGGT2 , and AsGGT3 , fused with the sequence encoding hexa-Gly residues, were cut out as Sal I- Nco I fragments and were inserted between the Sal I and Nco I sites in pTH2 ( PHYLOGENETIC ANALYSIS Phylogenetic analysis was performed using MEGA version 6 software ( RESULTS IDENTIFICATION OF THREE GENES ENCODING -GLUTAMYL TRANSPEPTIDASES IN GARLIC We found two garlic EST clones, EPP005LLAA12S004013 and EPP005LLAA12S003688, that show high sequence homology with known GGTs in GarlicESTdb ( 2 )