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Fallone CA, Chiba N, van Zanten SV, Fischbach L, Gisbert JP, Hunt RH, Jones NL, Render C, Leontiadis GI, Moayyedi P, Marshall JK (2016) The Toronto consensus for the treatment of Helicobacter pylori infection in adults

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The culture supernatants were then discarded and replaced with complete medium containing 10% CCK-8 solution (Beyotime Biotechnology, Shanghai, China), according to the manufacturer's requirements, and the cells were incubated in a 37 C incubator in the dark for 1.5 h