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Membranes were incubated overnight at 4C with primary antibodies, then incubated with HRP-conjugated secondary Goat Anti-Rabbit or Goat Anti-Mouse IgG antibody (Bio-Rad, 1706515 and OBT1500P, Hercules, CA) at 1:2000 dilution for 1-2 hours at room temperature

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Different GLP-1 medications reach steady-state concentrations at different ratesliraglutide within approximately 3 days, dulaglutide within 2-4 weeks, and semaglutide within 4-5 weeks, according to their respective MHRA-approved prescribing information