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The mobile phase consisted of a linear gradient system of phase A (60% acetonitrile/40% water solution containing 10 mM ammonium acetate and 0.1% formic acid) and phase B (isopropanol:acetonitrile, 9:1, v/v): 0 min 60% A, 2 min 57% A, 12 min 40% A, 12.1 min 25% A, 18 min 1% A, 19 min 1% A, and 20 min 40% A

If youre eating far less because you simply arent hungry, it makes sense

This offers a longer shelf life, making it a practical option for those who use it regularly

If weight loss alone doesn't explain the benefit, inflammation may play an important role

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