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Electroporation After a 1-week acclimatization, cells were collected at a density of 1 10 6 cells and mixed with plasmid diluted in Opti-MEM reduced serum medium (Thermo Fisher Scientific)

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Polyamines like spermine and putrescine are involved in multiple biological processes, and their biosynthetic pathway in fungi is quite similar to that in animals

Due to the increase in stress in the weight-bearing part of the joint, long-term strain will lead to cartilage exfoliation, hyperosteogeny, synovial hyperplasia, degeneration, etc

Dexrazoxane also inhibits topoisomerase II activity (Figure 11B