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Experts say, Turning cyanocobalamin into its active forms is a complex process with many steps ( This complex process shows why its important to know how Vitamin B12 is metabolized

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Material and methods Materials Tamoxifen, 4-hydroxy-tamoxifen, Dimethylsulfoxide, 2,7-dichlorofluorescin diacetate, 5,5-dithio-bis (2-nitrobenzoic acid), colchicine, valspodar (PSC-833) and Tween-20 (P9416) were all purchased from Sigma Aldrich

To determine urinary creatine, 200 l urine were diluted 1:25 in 48 ml distilled water and processed as described above

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