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The exact amount of proteins was resolved on 420% SDSPAGE gel for separation and was electroblotted onto 0.22-m PVDF membranes (Merck-Millipore, CA, USA)

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To determine which would be the focus of this study, through immunoblotting and quantitative real-time PCR, the expression profiles of seven different classes of GSTs were explored at both the transcription and protein levels for 13 miscellaneous human cancer cell lines consisting of A549 (lung), MCF7 and MDA-MB-231 (breast), PANC-1, MIA Paca-2 and SW1990 (pancreas), M7609, COLO320HSR, SW480 and HCT116 (colon), HepG2 (liver), HT1080 (fibrosarcoma), and HeLa (cervical) were explored