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Be sure to drink plenty of water to keep things moving

Oral formulations require fasting and careful timing but deliver efficacy within 510% of injectable versions in most patients

After treatment with the blocking solution (0.3% Triton-X and 3% BSA in 0.01 M PBS) to block non-specific staining and increase permeability to antibodies, the brain sections were incubated with anti-c-Fos rabbit polyclonal antibodies (abcam) in the blocking solution at 4 C overnight

Slowly pour the water over the bones and vegetables, adding more as needed to make sure theyre fully submerged

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