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Colonic cells positive for INSL5, 5-hydroxytryptamine (5-HT, serotonin), GCG and GFP (for details of antibodies used, see Electronic supplementary material [ESM] Table 1) staining were manually counted and colocalisation assessed using a CellDiscoverer7 (Zeiss, Germany) and imaged using an SP8 confocal microscope (Leica Microsystems, Germany) with a 63 objective lens

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RDN plays a critical role in counseling this population to improve nutrient intake and support individuals on a weight loss journey