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The second strategy consisted of two PCR rounds performed each in a 50 l reaction volume with 5 l cDNA, 5 l Pfu DNA polymerase 10 buffer, 1.5 U Pfu DNA polymerase (Promega, Mannheim, Germany), 0.25 mM dNTPs, and 0.3 M primers

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Physicians will typically require documentation demonstrating a diabetes diagnosis, like an A1C level of 6.5 or higher, to prescribe Mounjaro

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