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Samples were separated using a Raptor HILIC-Si 2.1 100 mm, 2.7 m LC column (Restek) with a constant flow rate of 400 l/min following a flow gradient starting at 5% Mobile Phase B (H 2 O + 5 mM AmFm +0.5% formic acid) then going up to 40% in 5 min, which was followed by re-equilibration at 5% B for 4 min and 30s
The most significant source of Lcarnitine in the human diet is meat
After being thoroughly briefed on the study's objectives, procedures, and potential risks, all participants were informed and consented
However, the question of whether the benefits observed in rats might also apply to humans will not be easy to determine
