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Current opinion in biotechnology, 2003, 14(5): 533- 537

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IGF-1 LR3 (Insulin-like Growth Factor-1 Long R3) is a modified form of IGF-1 with enhanced receptor binding and extended half-life

Briefly, we extracted protein from LV tissues and performed immunoblotting for various proteins using the following primary antibodies: SERCA2a, NCX1 (Thermo Fisher Scientific), PLN-P Ser16 , total PLN (Badrilla Ltd), Akt-P Ser473 , Akt-P Thr308 , total Akt (Cell Signaling), AMPK-P Thr172 and total AMPK (Cell Signaling) and subsequently incubated with HRP conjugated secondary antibodies respectively

Ensuring you get enough B12 through diet or supplements is important for overall health