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Generation and quantification of persulfides on Cofilin1 Purified full-length human recombinant Cofilin 1 protein expressed in Escherichia coli (Abcam #62958) (11 g/sample) was incubated with GSH (1 mM), MitoPerSulf (100 M) or both in 50 mM HEPES buffer (pH 7.4) for 7 min at RT, followed by alkylation with IAM-TPP (20 mM) at RT for 30 min in the dark

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Severe TBI from blast has been associated with a greater incidence of traumatic vasospasm (Armonda et al., 2006) than severe nonblast TBI