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For cells, they were first washed with PBS and fixed at room temperature for 15 min, followed by incubation with the staining solution at pH 6.0 overnight at 37 C

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Combination dosing produced greater reductions in body weight than semaglutide alone over 20 weeks, with a tolerability profile broadly consistent with each component

doi: 10.1038/d41586-018-05719-4 37 SerOPovovJMekIPekLJanoutV

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