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Cells were cultured in RPMI-1640 media with 11 mM D-glucose (Gibco) supplemented with 10% fetal bovine serum (FBS), 10 mM HEPES, 1 mM sodium pyruvate, 50 M -mercaptoethanol, and 1% penicillin/streptomycin at 37C in a 95% O 2 / 5% CO 2 incubator

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Iranian Journal of Basic Medical Sciences, 20 (5), 557-568

The functions of amylin are complementary to insulin and to GLP-1 : Slows gastric emptying , similarly to GLP-1, but more strongly Suppresses glucagon , helps control postprandial glycemia Induces satiety , via the area postrema in the brainstem Regulates weight balance , long-term effect In type 2 diabetes amylin secretion deteriorates in parallel with insulin secretion , as the disease progresses, the -cells lose the ability to produce both hormones at once

Retroperitoneal melanocytic schwannoma