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Chronic stress elevates cortisol, which directly suppresses glutathione synthesis via inhibition of gamma-glutamylcysteine synthetase (GCS), the rate-limiting enzyme in glutathione production

2003 Nov-Dec;32(6):515-23

Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

While these supplements work from the inside out, excessive UV exposure is the number one cause of collagen breakdown

Reduced TXN orchestrates the reduction in oxidized cysteines found in numerous proteins influenced by ROS