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Subsequently, proteins were digested using 1:100 (enzyme: protein) LysC (Wako sequencing grade, 125-05061) at 37 C for 4 hours, followed by dilution with HPLC water to make a 1.5 M final urea concentration and digestion with 1:100 Trypsin (Promega sequencing grade, V5111) for 16 hours

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Diabetes (2004) This review article examined the scientific rationale and clinical evidence for amylin agonist therapy in diabetes management, establishing the conceptual framework for understanding pramlintide's unique role in metabolic disease treatment