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By eight weeks after treatment cessation, animals returned to their baseline weight

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That could mean that among elite athletes, the drugs might have significant effects for only a small group, including Williams, who are seeking to get back into shape

They found that most of the remaining cost sits in the longterm maintenance phase, so they argue that smarter, more affordable weightmaintenance strategies could make a real difference

The protein concentration was determined using the BCA Protein Assay Kit (Pioneer, Shanghai, China), and then the protein samples were transferred onto PVDF membranes by SDS-PAGE electrophoresis and then incubated with specific primary antibodies at 4 C overnight