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For this assay, 96-well Nunc micro-well plate (Thermo Fisher Scientific) was coated with purified human v3 integrin protein (200 ng of protein/well in PBS containing 1 mM MnCl 2 ) 35,63

Lactate dehydrogenase (LDH) release, adenosine triphosphate (ATP) level, iron ion, and lipid peroxidation (LPO) content were detected by commercial kits

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