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In addition to the regulation of 1 C metabolism, emerging evidence shows that enzymes in the one-carbon metabolic network also have important non-canonical functions, commonly referred to as moonlighting functions [5]

Cells were grown at 37 C to an optical density at 600 nm (OD 600 ) of 0.70.8 in lysogeny broth (LPS solution LB-05) medium, and then 0.5 mM of isopropyl-1-thio--D-galactopyranoside (LPS solution IPTG025) was added

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[15] This process of mitochondrial damage through energy overproduction is referred to as mitochondrial overheating

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