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Akt activation by Ca(2+)/calmodulin-dependent protein kinase kinase 2 (CaMKK2) in ovarian cancer cells

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Researchers at the University of Texas first characterized 5-amino-1MQ (5-amino-1-methyl quinolinium) in 2017 while searching for ways to inhibit nicotinamide-N-methyltransferase (NNMT) [1]

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Reactive oxygen species (ROS) detection assay For C11-BODIPY staining, the C11-BODIPY reagent was added to the cell suspension to achieve a final concentration of 1 to 10 M, followed by incubation at 37 C for 30 to 60 min