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What Results Should Patients Expect

A decrease in 5,10-methylene THF can compromise the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) by the enzyme thymidylate synthase (TYMS), causing uracil accumulation and thymine depletion

10.1073/pnas.242594999 216 SteinerT

Protein overexpression was induced by adding 0.5 mM isopropyl -D-1-thiogalactopyranoside (IPTG) to bacterial cultures grown at 37C until an OD 600nm of 0.6

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