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(2)

CHO-SNAP-GLP-1R cells were pretreated 100 nM bafilomycin to generate neutral endosomal pH 30 and exposed to 100 nM FITC-agonists for 30 min at 37 C to induce internalization

When both receptor families are activated at the same time, several experimental datasets suggest that growth hormone output may rise beyond what is seen with either pathway alone, which is compatible with additive or synergistic coupling at the level of pituitary signaling

see also Section 2.8), following the datasheet recommendations

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