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Reconstitute with sterile bacteriostatic water or appropriate laboratory buffer Add solvent slowly along the vial wall to minimize foaming Gently swirl until the peptide is dissolved Store reconstituted solutions at 3646 F (28 C) Prepare aliquots where appropriate to reduce freezethaw cycles Careful preparation procedures help maintain peptide stability and solution consistency during laboratory experiments

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In addition to functional analysis, the mitochondrial DNA copy number was measured via quantitative PCR to determine mitochondrial biogenesis

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Dosage may vary depending on species, weight, and health condition