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The Ransod assay (RANDOX Laboratories, Crumlin, UK) was used to quantify the activity of superoxide dismutase (SOD), which is an antioxidant enzyme that protects cells against the pro-oxidant action of superoxide free radicals

Broiler chicks supplemented Se via sodium selenite had greater expression of selenoprotein N1, W1 , and O in the pectoral muscle compared to non-supplemented chicks ( Sepw1 expression was highly regulated within muscle with sodium selenite and Se-deficiency ( Selenoprotein H Selenoprotein H was initially identified using bioinformatics methods developed around selenocysteine insertion sequence (SECIS) elements of human genomes ( via Western blot and immunohistochemistry, SELENOH was found in the nucleolar fraction specifically within the nucleoli ( SELENOH expression in neuronal cells appears to play a pivotal role in mitochondrial biogenesis and respiratory capacities

This triggers an immediate, strong pulse of growth hormone release

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