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Sample handling: Analysts must meticulously control temperature, dilution, and buffer exchange to avoid artifactual aggregation, ensuring SEC accurately reflects the drug's structural integrity

Left gel shows very little GST-fusion protein in the flow-through and a lot in the elution indicating that sufficient glutathione agarose resin was used in this purification

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Mechanistically, high-glucose conditions induced robust upregulation of BMP4 and increased phosphorylation of its downstream effectors Smad1/5/9, driving tumorigenic signaling