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Cells were washed three times with ice-cold PBS and lysed in lysis buffer (40 mM Tris-HCl (pH 8.0), 1% Triton X-100, 1% NP-40, 10% glycerol, 0.15 M NaCl, 2 mM EDTA, 1 mM phenylmethanesulfonyl fluoride, 1 Complete protease inhibitor cocktail)

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Better Sleep on GLP-1s: How to Get the Rest You Need In This Article If you're on a GLP-1 medication like Ozempic, Wegovy, Mounjaro, or Zepbound, you might have noticed changes in your sleep patterns

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