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In summary, this study revealed that dysfunction of cellular transport of cytoplasmic FA to mitochondria plays a critical role in UA-induced cardiomyocyte injury and thereby promotes LVR development and identified CPT1B as the key factor influenced by UA

Fura-2 assay for detection of cytosolic [Ca 2+ ] HEK293 cell monolayers transfected with either NPY2R or the EV were loaded with the fluorescent Ca 2+ indicator Fura-2-AM so that real-time kinetic assays of cytosolic [Ca 2+ ] could be performed in a 96-well format using a Flexstation 3 microplate reader, as described previously 28

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