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Drawing exactly 12.5 units requires estimating the halfway point between markings

For detailed protocols matching published research standards, our peptide storage guide provides specific guidance on aliquoting, handling, and shelf-life considerations used in laboratory research

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The NbLumSyt1-HALO/JF549 complex was then centrifuged at 18.000 g for 10 min at RT to remove precipitates, and subsequently diluted (1:200) in High K + buffer, mixed at RT for 5 min in gentle rotation (20 rpm), and used for imaging on the same day