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At optimal concentrations, GHK-Cu increases mRNA production for collagen types I and III, elastin, proteoglycans, and glycosaminoglycans, while laboratory studies show that topical application for 12 weeks improves collagen production in 70% of subjects compared to 50% with vitamin C and 40% with retinoic acid

Energy minimization was conducted with the steepest descent method (maximum force tolerance of 1000 kJmolnm, step size 0.01 nm), followed by 100 ps NVT equilibration (0310.15 K) and 100 ps NPT equilibration (310.15 K, 1 bar) using the Berendsen barostat