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For the global metabolite profiling in serum, the metabolites in 10 L of serum were extracted by the 390 L of pre-chilled 82% MeOH (80 C) followed by 15 min incubation at 80 C

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GSH plays roles in detoxifying toxic compounds, protein folding, the regeneration of vitamins C and E, maintenance of mitochondrial function, antiviral defense, the regulation of cellular proliferation, apoptosis, and the immune response