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Primary rat hepatocytes (PRH) Male Wistar rats (Janvier Labs, Le Genest-Saint-Isle, France) were used to isolate PRHs by in situ liver perfusion

Glutathione S-transferase (GST) can be used as a control for GST-fusion tag proteins

Before making the diagnosis of PFIC, in the paediatric population, other cholestasis disorders should always be considered in the differential diagnosis, including biliary atresia, choledochal malformations, congenital endocrine disorders, inborn errors of metabolism (mainly during the neonatal period) as well as autoimmune liver diseases, drug-induced cholestasis and other genetic diseases in older children [72]