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Administering the treatment slowly (over the course of around 30 minutes) can help avoid this unwanted effect
*Correspondence: Lian-Shun Wang, [email protected] This article was submitted to Aquatic Physiology, a section of the journal Frontiers in Physiology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
After centrifugation, input samples were taken, and the remaining lysates were diluted in the dilution buffer (0.01% SDS, 1% triton, 2 mM EDTA, 20 mM tris pH 8,1, 150 mM NaCl)
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