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Finally, isolated neovascularized corneas were extracted in a cold lysis buffer composed of protease and phosphatase inhibitors, and prepared for Western Blot (WB) assays with primary antibodies for Anti-MMP2 (ab92536, Abcam, Cambridge, UK), Anti-MMP9 (ab76003, Abcam), and Anti-VEGFR2 (ab39638, Abcam)