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The culture was split into two 300 ml cultures and each flask was diluted to 1.0 10 6 cells/mL, usually leading to a final volume of 600 mL that complies with the ratio of air to cell medium of 4:1

Several studies undertaken globally show that women with vitamin B12 deficiency in early pregnancy were up to five times more likely to have a child with neural tube defects, such as spina bifida, compared to women with high levels of vitamin B12

The released fatty acids can then be used by the body for energy metabolism

While methylcobalamin may be more readily absorbed than synthetic types, research has not shown a significant difference among the various vitamin B12 types

Additionally, a bicinchoninic acid (BCA) assay was used to determine the protein concentration